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Size Matters: Metabolic Rate and Longevity John Tukey once said, “The best thing about being a statistician is that you get to play in everyone’s backyard.” I enthusiastically agree! I frequently enjoy reading and watching science-related material. This invariably raises questions, involving other “backyards,” that I can better understand using statistics. For instance, see my […] The summary of tophat-cufflinks protocol is like that: step1: generate a tophat_out folder with bam files tophat -G genes.gtf <index> sample1_1.fq sample1_2.fq tophat -G genes.gtf <index> sample2_1.fq sample2_2.fq step2: generate new .gtf files (assemble isoform) cufflinks sample1/accepted_hits.bam cufflinks sample2/accepted_hits.bam step3: prepare a text file named assemblies.txt with following gtf files cat << EOF > assemblies.txt […] |
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