Vipie: web pipeline for parallel characterization of viral populations from multiple NGS samples

Background

Next generation sequencing (NGS) technology allows laboratories to investigate virome composition in clinical and environmental samples in a culture-independent way. There is a need for bioinformatic tools capable of parallel processing of virome sequencing data by exactly identical methods: this is especially important in studies of multifactorial diseases, or in parallel comparison of laboratory […]

September 16th, 2019 | Category: 二代测序, 生物信息 | Leave a comment

Utilities / Create consensus sequence from a BAM file

Utilities / Create consensus sequence from a BAM file Description

Given an indexed BAM file and corresponding reference genome (in fasta format), this tool constructs a consensus sequence based on the alignment.

Parameters

None

Details

This tool uses SAMtools, bcftools and vcfutils.pl script to create a consensus sequence for the given alignment file. The actual […]

September 9th, 2019 | Category: 二代测序 | Leave a comment