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熟悉我们生信技能树团队的应该都知道大名鼎鼎的y叔啦，作为我们论坛的荣誉顾问，y叔一直勤勤恳恳的指出我们的错误，特意在此谢谢y叔！并奉上y叔的ChIP-seq数据分析大礼包，已经征得y叔同意啦！ 关注Y叔微信公众账号biobabble CS0: ChIPseq从入门到放弃 接下来要出一个ChIPseq系列，讲一讲ChIPseq和我的 […] https://guangchuangyu.github.io/2014/04/chipseeker-for-chip-peak-annotation/ ChIPpeakAnno WAS the only R package for ChIP peak annotation. I used it for annotating peak in my recent study. I found it does not consider the strand information of genes. I reported the bug to the authors, but they[……] […] For details, read http://beast2.cs.auckland.ac.nz/index.php/Main_Page and http://beast2.cs.auckland.ac.nz/index.php/FAQ . For any problem, do not hesitate to browse through the list of questions on the BEAST forum https://groups.google.com/forum/#!forum/beast-users .
Exercise description[……]
[…] 何謂ChIP-Seq? ChIP–seq ( Chromatin immunoprecipitation sequencing )是指染色質免疫沉澱後，所獲得的DNA片段進行高通量定序，並將此片段利用生物資訊的軟體對回至基因體，可以瞭解DNA-binding proteins及histone modifications的狀況，進而得知染色質及相结合的調控因子之間的相互作用關係。 ChIP-chip與ChIP-Seq差異? 次世代定序較ChIP-chip提供更高的解析度，較少的雜訊，較少的ChIP-DNA的量，及可偵測的動態範圍及基因體範圍較廣，因此可呈現較真實的基因調控及表觀[……] […] In this tutorial, we’re going to take a set of Illumina reads from an inbred Drosophila melanogaster line, and map them back to the reference genome. (After these steps, we could do things like generate a list of SNPs at which this line differs from the reference strain, or generate a genome sequenc[……] […] |
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