MetaPhlAn: Metagenomic Phylogenetic Analysis

MetaPhlAn is a computational tool for profiling the composition of microbial communities from metagenomic shotgun sequencing data. MetaPhlAn relies on unique clade-specific marker genes identified from 3,000 reference genomes, allowing:

  • up to 25,000 reads-per-second (on one CPU) analysis speed (orders of magnitude faster compared to existing methods);
  • unambiguous taxonomic assignments as the MetaPhlAn markers are clade-specific;
  • accurate estimation of organismal relative abundance (in terms of number of cells rather than fraction of reads);
  • species-level resolution for bacterial and archaeal organisms;
  • extensive validation of the profiling accuracy on several synthetic datasets and on thousands of real metagenomes.

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