454 pyrosequencing analysis pipeline

mothur > sffinfo(sff=454Reads_archaea.sff, flow=T) Extracting info from 454Reads_archaea.sff … 10000 20000 30000 40000 50000 60000 70000 80000 90000 92115 It took 68 secs to extract 92115. Output File Names: 454Reads_archaea.fasta 454Reads_archaea.qual 454Reads_archaea.flow

mothur > trim.flows(flow=454Reads_archaea.flow, oligos=oligos_LXY.txt, pdiffs=2, bdiffs=1, processors=2) Appending files from process 15674

Output File Names: 454Reads_archaea.trim.flow 454Reads_archaea.scrap.flow 454Reads_archaea.GZ_ARC.flow 454Reads_archaea.GZ1122_ARC.flow 454Reads_archaea.GZ1122cellulose_ARC.flow 454Reads_archaea.GZ_xylan_ARC.flow 454Reads_archaea.GZ_cellulose55_ARC.flow 454Reads_archaea.SHX_xylan_ARC.flow […]