Amoa project
A2,A3 amoa sample sequencing
(1) use geneious to deal with the sequence raw data, get 635bp DNA sequence
(2)use mafft to get the alignment results
(3)mothur deal pipline
mothur > dist.seqs(fasta=AMOA_A2A3.mafft.align.shortname.fasta, output=lt)
0 0 59 0
Output File Name: AMOA_A2A3.mafft.align.shortname.phylip.dist
It took 0 to calculate the distances for 60 sequences.
mothur > cluster(phylip=AMOA_A2A3.mafft.align.shortname.phylip.dist, […]
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