生物資訊教學( Good sources)

生物資訊教學

生物資訊是一門整合生命科學與電腦科學的跨領域研究。此Wiki由【計算生物學】與【生物資訊演算法】之修課學生,國立中正大學生物資訊實驗室同學,與指導老師黃耀廷博士所共同編寫。生物資訊之背景知識,涵蓋部份生物、遺傳、統計、與演算法之背景知識,可分以下子課題介紹:

生命科學 遺傳演化 生物統計 生物資訊演算法 Assembly Tool: 沒有參考基因體(Reference Genome)的物種(例如,鴿子、蓮霧),定序完必須把破碎的序列重組出一條參考基因體。 Assembly Tools: de bruijn graph assemblers: Velvet、Euler-SR、SOAPdenovo、ABySS、CLCBio Overlap-graph assemblers: MIRA、AMOS package Scaffolder: SOAPdenovo, SSPACE, Bambus, IMAGE2. Gene Prediction Gene Ontology Annotation Alignment Tool: 已經有參考基因體的物種(例如,人類、稻米),定序完可以直接把序列對回去(Align)參考基因體。 Short read alignment: BOWTIE、BWA、Bfast、SOAPaligner、MAQ Alignment檔案處理: SAMTools、Tabix for range query、Split Bam、挑出成對序列 Local realignment for small indels: SAMTools、GATK Long read alignment: BLAT、BLAST使用範例、wwwblast安裝設定、Lastz […]

gene prediction pipeline

augustususe EST file blat -minIdentity=92 aphis_genome_scaffold_v3.1.fa aphis-unigene.idx.fna aphis-unigene.idx.psl /home/soft/pslCDnaFilter -maxAligns=1 aphis-unigene.idx.psl aphis-unigene.idx.f.psl /home/soft/augustus.2.5/scripts/blat2hints.pl –in=aphis-unigene.idx.f.psl –out=hints.E.gff./augustusRun.pl /public/d_aphis/DNA_assembly/3.1fix/gene_predict/augustus aphis_genome_scaffold_v3.1.fa pea_aphid hints.E.gff 15./augustusRun.py -i aphis_genome_scaffold_v3.1.fa -n pea_aphid -j hints.E.gff -s 35000000 &# —– prediction on sequence number 3 (length = 1746996, name = AGO_S000003) —–geneid:./scripts/gffftablExport.pl aphis.geneid.model.gff3 aphis.geneid geneidblast2go-rw-r–r– 1 zhaoqy users 90672652 2010-10-15 gene2go-rw-r–r– 1 zhaoqy users 19294682 […]

Gene Prediction

Bacteria Gene Prediction

* GeneMark and Glimmer This provides the foundation for operon predictions and promotor predictions. One way to verify the gene prediction result is to check the presence of Shine-Dalgarno sequence in fron of each gene which is a purine-rich region with a consensus AGGAGG and is located within 20 bp upstream of […]