Command-line tools for processing biological sequencing data. Barcode demultiplexing, adapter trimming, etc.

http://code.google.com/p/ea-utils/

 

Primarily written to support an Illumina based pipeline – but should work with any FASTQs.

Overview: fastq-mcf

Scans a sequence file for adapters, and, based on a log-scaled threshold, determines a set of clipping parameters and performs clipping. Also does skewing detection and quality filtering.

fastq-multx

Demultiplexes a fastq. Capable of auto-determining barcode […]

Venn diagram online software

http://bioinformatics.psb.ugent.be/webtools/Venn/

An approximate workflow for repeating the phylogenetic analysis of strawberry

An approximate workflow for repeating the phylogenetic analysis of strawberry and other plant genomes would consist of the following steps: 1) Obtain protein and nucleotide sets from the identified sources. Extract subregions of protein and nucleotide sequences specified in the gene identifiers spreadsheet and group into files by family. 2) Search nucleotide sequences for papaya […]

网站版权copyright后面的符号输出显示

copyright是著作版权声明,一般出现在网站底部。 示例:Copyright © 1998 表示:在1998所有拷贝权(也就是使用权),所有的权利都给所有者使用。当然,著作版权声明还有好多其他的写法,你可以在网络上搜索到相关示例,这里就不啰嗦了。 但是Copyright © 1998 后面这个圈C的标记符号很多站长输入到网页中却无法输出显示出来,不是不识别,就是变成小点。 怎么办呢?问题的核心主要是这个圈C的符号字体编码设置不当,这里提供了几种copyright后面符号输出显示的方法: (1)DW中,插入-特殊符号-版权即可;</P> (2)或者直接在源代码中输入” &copy “; (3)在PS里,制作效果图的时候,会显示成小一点的,在网页中,如果字体为12PX的话,会显示正常的; (4)这是font-family的设置问题…只要把那些个字的font-family设为Arial, Helvetica, sans-serif就可以了

【转】PBS环境qsub, qstat, qdel(1)

PBS 是公开源代码的作业管理系统,在此环境下运行,用户不需要指定程序在哪些节点上运行,程序 所需的硬件资源由PBS 管理和分配。 1、PBS 命令 PBS 提供4 条命令用于作业管理。 (1) qsub 命令—用于提交作业脚本 命令格式: qsub [-a date_time] [-c interval] [-C directive_prefix] [-e path] [-I] [-j join] [-k keep] [-l resource_list] [-m mail_options] [-M user_list][-N name] [-o path] [-p priority] [-q destination] [-r c] [-S path_list] [-u user_list][-v variable_list] [-V] [-W additional_attributes] [-z] [script] 参数说明:因为所采用的选项一般放在pbs 脚本中提交,所以具体见PBS 脚本选项。 […]

High CPU/RAM usage and Parallel(MPI) jobs submitted sample scripts

for Single CPU Job

zyshen@e2lx001:/home/zyshen> cat /disk/rdisk03/test_1cpu.sh #!/bin/csh # # #PBS -l nodes=1:ppn=1 # #******** Script for job submission with single CPU #******** The lines above must be included in any job script #******** ‘nodes’ is number of nodes and ‘ppn’ is number of CPU per node, which are both ‘=1′ in this case. # […]